- Visibility 385 Views
- Downloads 384 Downloads
- Permissions
- DOI 10.18231/j.ijfmts.2025.012
-
CrossMark
- Citation
Saliva as a diagnostic tool in criminal investigation: A scoping review
Saliva is increasingly recognized as a valuable diagnostic tool in criminal investigations due to its potential for forensic analysis. As a bodily fluid, saliva contains DNA, enzymes, proteins, and other substances that can be used to identify individuals and gather crucial evidence in criminal cases.
One of the primary applications of saliva in forensic science is its role in DNA analysis. Saliva contains cells from the inner cheek and mouth lining, which carry unique DNA signatures. These cells can be collected from items like cigarette butts, bottles, or discarded tissues at crime scenes. DNA profiling of saliva can help link a suspect to a crime scene, provide evidence of contact, or exclude individuals from suspicion.
In addition to DNA analysis, saliva also contains biomarkers and substances that may be relevant in specific investigations. For example, the presence of certain drugs, alcohol, or toxins in saliva can provide evidence of intoxication or drug use, which may be pertinent in cases such as impaired driving or poisoning. Furthermore, certain enzymes or proteins found in saliva could help identify injuries or medical conditions that may be relevant in criminal investigations.
Saliva can also serve as trace evidence in cases of sexual assault or violent crimes. When collected properly, saliva left at a crime scene can provide critical evidence of contact between a victim and a perpetrator, aiding in the identification of the suspect.
Overall, saliva is a non-invasive and easily collectable substance that holds great potential as a diagnostic tool in criminal investigations, offering reliable, cost-effective methods to gather key forensic evidence.
Keywords: Saliva, Trace evidence, Criminal investigation, Forensic medicine
References
- Kapoor P, Chowdhry A. Salivary signature in forensic profiling: A scoping review. J Forensic Dent Sci. 2018;10(3):123–7.
- Humphrey SP, Williamson RT. A review of saliva: Normal composition, flow, and function. J Prosthet Dent. 2001;85(2):162-
- Edgar, WM. Saliva: its secretion, composition and functions. Br Dent J. 1992;172(8):305-12.
- Levine, MJ. Salivary macromolecules. A structure/function synopsis. Ann NY Acad Sci. 1993;20;694:11-6.
- Vajagathali M., Sudhan M., Vignesh S., Jayashree T.S., and Moorthi A. Standardized protocol for the isolation of DNA from human saliva- a pilot study. Int Res J Biol Sci.10(3);(2021):40-4.
- Kame'enui EJ, Simmons DC. Introduction to this special issue: The DNA of reading fluency. Sci Stud Read, 2001;5(3);203-10.
- John SJ, Rajaji D, Jaleel D, Mohan A, Kadar N, Venugopal V. Application of Saliva in Forensics. Oral Maxillofac Pathol J. 2018;9(2):85-7.
- Aps JK, Martens LC. Review: the physiology of saliva and transfer of drugs into saliva. Forensic Sci Int. 2005;150(2-3):119-31.
- Sweet D, Lorente M, Lorente JA, Valenzuela A, Villanueva E. An improved method to recover saliva from human skin: the double swab technique. J Forensic Sci. 1997;42(2):320-2.
- Saxena S, Kumar S. Saliva in forensic odontology: a comprehensive update. J Oral Maxillofac Pathol. 2015;19(2):263-5.
- Chatterjee S. Saliva as a forensic tool. J Forensic Dent Sci. 2019;11(1):1–4.
- Dawnay N, Sheppard K. From crime scene to courtroom: A review of the current bioanalytical evidence workflows used in rape and sexual assault investigations in the United Kingdom. Sci Just. 2023;63(2):206–28.
- Verma AK. Analysis and Identification of Bite Marks in Forensic Odontology-A Review Article‖. 2022;5(4):28–37.
- MacDonald DG. Bite mark recognition and interpretation. J Foren Sci Soc. 1974;14(3):229-33.
- Furness J. A new method for the identification of teeth marks in cases of assault and homicide. Brit Dent J. 1968;124(6):261-7.
- Schipper RG, Silletti E, Vingerhoeds MH. Saliva as research material: Biochemical, physicochemical and practical aspects. Arch Oral Biol. 2007;52(12);1114-35.
- Huang C-M. Comparative proteomic analysis of human whole saliva. Arch Oral Biol. 2004;49(12):951-62.
- Bum J K, Han C, Moon H, Kwon J, Ik-Soon Jang, Si-Keun Lim, Ki- Won Park, Jong-Soon Choi & Hyun Joo An. Monitoring of post- mortem changes of saliva N-glycosylation by nano LC/MS. Anal Bioanal Chem.2018;410:45–56.
- Mago J, Sidhu L, Kaur R, Kamal, Anurag T. Saliva in forensics. World J Pharm Med Res. 2016;2(4):196-8.
- Yamuna PK, Prathibha MK. Methods of collection of saliva - A Review. Int J Oral Health Dent. 2017;3(3):149-53.
- Navazesh M. Methods for collecting saliva. Ann New York Acad Sci.1993;694(1):1-1.
How to Cite This Article
Vancouver
Singh G, Mulay AA. Saliva as a diagnostic tool in criminal investigation: A scoping review [Internet]. IP Int J Forensic Med Toxicol Sci. 2025 [cited 2025 Oct 03];10(2):41-43. Available from: https://doi.org/10.18231/j.ijfmts.2025.012
APA
Singh, G., Mulay, A. A. (2025). Saliva as a diagnostic tool in criminal investigation: A scoping review. IP Int J Forensic Med Toxicol Sci, 10(2), 41-43. https://doi.org/10.18231/j.ijfmts.2025.012
MLA
Singh, Gambhir, Mulay, Avanie Atul. "Saliva as a diagnostic tool in criminal investigation: A scoping review." IP Int J Forensic Med Toxicol Sci, vol. 10, no. 2, 2025, pp. 41-43. https://doi.org/10.18231/j.ijfmts.2025.012
Chicago
Singh, G., Mulay, A. A.. "Saliva as a diagnostic tool in criminal investigation: A scoping review." IP Int J Forensic Med Toxicol Sci 10, no. 2 (2025): 41-43. https://doi.org/10.18231/j.ijfmts.2025.012